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Serious leaking my sources

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negativ said:
gimme a hgh source
Click to expand...
Do you mean HGH (human growth hormone) from a doctor/prescription or an evidence source about HGH?

If you want a reliable evidence source, try: Endocrine Society clinical practice guideline on growth hormone deficiency and hormone replacement.
 
trvecel said:
Do you mean HGH (human growth hormone) from a doctor/prescription or an evidence source about HGH?

If you want a reliable evidence source, try: Endocrine Society clinical practice guideline on growth hormone deficiency and hormone replacement.
Click to expand...
gimme a hgh source
 
negativ said:
gimme a hgh source
Click to expand...
Step 1: Design the Amino Acid Sequence
Human growth hormone (hGH) consists of 191 amino acids. The exact sequence is known and can be found in databases like the Human Genome Project or the Protein Data Bank (PDB). This sequence will be used to guide the synthesis process.

Step 2: Choose a Solid Support
In solid-phase peptide synthesis (SPPS), a solid support such as a resin (e.g., Wang resin or Rink amide resin) is used as a base to which the first amino acid will be attached. The resin is chosen based on the desired final product (e.g., N-terminal acetylation or C-terminal amide).

Step 3: Attach the First Amino Acid
The first amino acid (usually N-terminal) is attached to the resin using a coupling agent, such as dicyclohexylcarbodiimide (DCC) or N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide (EDC). The amino acid is typically in the form of a protected amino acid (e.g., with a tert-butyl group to block the amino group).

Step 4: Sequential Addition of Amino Acids
The remaining amino acids are added one at a time in the correct sequence. Each amino acid is activated using a coupling agent and then attached to the growing peptide chain on the resin. The side chains of the amino acids are protected to prevent unwanted reactions during the synthesis.

Step 5: Deprotection of Amino Acids
After each amino acid is added, the protecting groups on the amino acid side chains are removed (deprotected) using an appropriate reagent, such as trifluoroacetic acid (TFA) or another deprotecting agent. This allows the next amino acid to be added to the chain.

Step 6: Cleavage from the Resin
Once the entire sequence of 191 amino acids is added, the peptide is cleaved from the resin. This is typically done using a cleavage reagent like TFA, which also removes any remaining protecting groups from the peptide chain.

Step 7: Purification
The synthesized growth hormone is purified using techniques such as high-performance liquid chromatography (HPLC), ion exchange chromatography, or reverse-phase chromatography. These methods help to remove any byproducts, unreacted amino acids, and other impurities.

Step 8: Final Refinement and Quality Control
The purified growth hormone is further refined and analyzed using mass spectrometry (MS) or nuclear magnetic resonance (NMR) to confirm its structure and purity. This step ensures the hormone is biologically active and free of contaminants.

Step 9: Formulation and Storage
The purified growth hormone is then formulated into a suitable solution, often in a buffer such as phosphate-buffered saline (PBS), and stored under appropriate conditions (e.g., refrigerated) to maintain its stability and activity.

This chemical synthesis method is used in laboratories and pharmaceutical industries to produce recombinant human growth hormone for medical and research purposes.
 
trvecel said:
Step 1: Design the Amino Acid Sequence
Human growth hormone (hGH) consists of 191 amino acids. The exact sequence is known and can be found in databases like the Human Genome Project or the Protein Data Bank (PDB). This sequence will be used to guide the synthesis process.

Step 2: Choose a Solid Support
In solid-phase peptide synthesis (SPPS), a solid support such as a resin (e.g., Wang resin or Rink amide resin) is used as a base to which the first amino acid will be attached. The resin is chosen based on the desired final product (e.g., N-terminal acetylation or C-terminal amide).

Step 3: Attach the First Amino Acid
The first amino acid (usually N-terminal) is attached to the resin using a coupling agent, such as dicyclohexylcarbodiimide (DCC) or N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide (EDC). The amino acid is typically in the form of a protected amino acid (e.g., with a tert-butyl group to block the amino group).

Step 4: Sequential Addition of Amino Acids
The remaining amino acids are added one at a time in the correct sequence. Each amino acid is activated using a coupling agent and then attached to the growing peptide chain on the resin. The side chains of the amino acids are protected to prevent unwanted reactions during the synthesis.

Step 5: Deprotection of Amino Acids
After each amino acid is added, the protecting groups on the amino acid side chains are removed (deprotected) using an appropriate reagent, such as trifluoroacetic acid (TFA) or another deprotecting agent. This allows the next amino acid to be added to the chain.

Step 6: Cleavage from the Resin
Once the entire sequence of 191 amino acids is added, the peptide is cleaved from the resin. This is typically done using a cleavage reagent like TFA, which also removes any remaining protecting groups from the peptide chain.

Step 7: Purification
The synthesized growth hormone is purified using techniques such as high-performance liquid chromatography (HPLC), ion exchange chromatography, or reverse-phase chromatography. These methods help to remove any byproducts, unreacted amino acids, and other impurities.

Step 8: Final Refinement and Quality Control
The purified growth hormone is further refined and analyzed using mass spectrometry (MS) or nuclear magnetic resonance (NMR) to confirm its structure and purity. This step ensures the hormone is biologically active and free of contaminants.

Step 9: Formulation and Storage
The purified growth hormone is then formulated into a suitable solution, often in a buffer such as phosphate-buffered saline (PBS), and stored under appropriate conditions (e.g., refrigerated) to maintain its stability and activity.

This chemical synthesis method is used in laboratories and pharmaceutical industries to produce recombinant human growth hormone for medical and research purposes.
Click to expand...
i changed my mind, i want a ghrp source, its cheaper (dont ask how)
 
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