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Blood fillers, and how you can make your ownÂŽ
Did you know that fat grafting isnât the only filler method that uses your own autologous material?
Ever heard of EZ gel?
Or the vampire facial?
Let me introduce you to blood filler, also known as âvampire fillersâ. Itâs pretty self explanatory as to what it is. They extract blood from you and make it into a filler. I researched and figured out how you can make them yourself.
What are the benefits of doing this yourself?:
-Itâs your OWN BLOOD, meaning unlike regular fillers you have a lifetime supply of it in your own body. Very cost efficient.
-Because itâs your own blood, there is practically 0 risk of allergies, inflammation, and most of that crap
-Increases growth factors, collagen synthesis, tissue remodeling
-It can last up to a year
Cons:
-Itâs not permanent, shocker
-provides limited structural support
-takes multiple sessions for optimal results
Kinds of blood fillers:
PRP
This stands for platelet rich plasma. Now what the hell does that mean? Plasma and platelets are both found in your blood. Plasma meaning the yellow liquid base that makes up ~55% of your blood, and platelets meaning blood cells that repair and rejuvenate. Now that thatâs explained, you can guess what this treatment is about.
This treatment is used in orthopedic contexts that help to heal muscles, tendons, and more, but it has also grown popular in the cosmetic industry. PRP injections have been used for collagen stimulation, scar covering, skin rejuvenation, AND hair rejuvenation. It is injected and can also be microneedled into the face, which is called the âvampire facialâ treatment. Using your own blood to regenerate your youthfulness.
PRF
This on the other hand stands for platelet rich fibrin. This isnât like PRP where itâs liquidy, this one is stickier and is used as a filler. Fibrin is a sticky protein found in your blood that almost acts as a net and traps platelets and other blood cells. Itâs used for basically the same things as PRP, but the unique part about it is its filler type consistency. If youâve heard of EZ gel, this is what is used to make it. PRF can also be used for a vampire facial, or just injected by itself.
I was thinking about adding a PRFM one, but calcium chloride 10% is unfortunately not available to buy anywhere
Iâm not a medical professional, this post is not encouraging anybody to replicate what Iâve said. The information here is all purely based off research,
@Chainsandwhips2 @arya @TonyDr @genio @foidslayer @Histy @LaWi @ecoli
Did you know that fat grafting isnât the only filler method that uses your own autologous material?
Ever heard of EZ gel?
Or the vampire facial?
Let me introduce you to blood filler, also known as âvampire fillersâ. Itâs pretty self explanatory as to what it is. They extract blood from you and make it into a filler. I researched and figured out how you can make them yourself.
What are the benefits of doing this yourself?:
-Itâs your OWN BLOOD, meaning unlike regular fillers you have a lifetime supply of it in your own body. Very cost efficient.
-Because itâs your own blood, there is practically 0 risk of allergies, inflammation, and most of that crap
-Increases growth factors, collagen synthesis, tissue remodeling
-It can last up to a year
Cons:
-Itâs not permanent, shocker
-provides limited structural support
-takes multiple sessions for optimal results
A centrifuge is vital to this process. Itâs a device that will spin your blood samples at high speeds. Its magic is that by spinning your samples its force will settle the layers of the blood, and separate them from heavy at the bottom to lighter at the top.
If youâve heard of this device already, youâre probably thinking how the fuck am I supposed to pay for this? This is an expensive device.
Well thatâs exactly what I assumed in the beginning to, until I looked deeper and found cheap ones. They sell these perfectly ideal centrifuges on eBay, and Amazon for 60 bucks at most. The models speed ranges up until 4000rpm, its max force is 1790g, the rotor radius is ~10, and can hold 20ml test tubes. Itâs perfect.
https://www.ebay.com/itm/3242313841...775&gbraid=0AAAAAD_QDh-htVzFNlS6O5UqN1tEvprpg
Centrifuges come in different kinds, thereâs fixed angled and horizontal. Horizontal is proven to be superior, but thereâs no cheap horizontal centrifuge out there in sight. Beggars canât be choosers. We will be using fixed.
The centrifuge I listed offers 6 holes, it doesnât mean you need to fill each one but with the ones you do fill it must be even. You need to have a counterbalance. For example, if you stick a full 10cc tube of blood in one hole, you must stick another 10cc tube thatâs full right across from it. They must have the same weights for this to work properly, so some people will even fill up an identical tube with water or something just to add weight where itâs due.
In this pic I made, the red represents a filled tube. You see how if you add it in one hole, you need to add another evenly filled one in the hole across from it. To add a counterbalance. If the centrifuge is unbalanced, itâll start shaking, making weird noises, and can ultimately damage the device.
Your centrifuge will have its own timer, so itâll shut itself off whenever you want. It also has a speed adjustment knob which will control the RPM. The max RPM is 4000, so each knob (beginning at zero), is probably ~666 RPM each.
There are a few different vocab words that you should know about the centrifuge.
RPM: rotations/revolutions per minute.
G-force/RCF: the term g force means gravity force, how much times stronger is the force than normal gravity? A higher g force would mean itâs spinning faster. This is expressed in g (ex: 100g would mean 100 times earths gravity).
Rotor: the rotor is the part of your centrifuge that spins.
Rotor radius: distance from the center. The greater distance from the center, the more increased your RCF will be.
If youâve heard of this device already, youâre probably thinking how the fuck am I supposed to pay for this? This is an expensive device.
Well thatâs exactly what I assumed in the beginning to, until I looked deeper and found cheap ones. They sell these perfectly ideal centrifuges on eBay, and Amazon for 60 bucks at most. The models speed ranges up until 4000rpm, its max force is 1790g, the rotor radius is ~10, and can hold 20ml test tubes. Itâs perfect.
https://www.ebay.com/itm/3242313841...775&gbraid=0AAAAAD_QDh-htVzFNlS6O5UqN1tEvprpg
Centrifuges come in different kinds, thereâs fixed angled and horizontal. Horizontal is proven to be superior, but thereâs no cheap horizontal centrifuge out there in sight. Beggars canât be choosers. We will be using fixed.
The centrifuge I listed offers 6 holes, it doesnât mean you need to fill each one but with the ones you do fill it must be even. You need to have a counterbalance. For example, if you stick a full 10cc tube of blood in one hole, you must stick another 10cc tube thatâs full right across from it. They must have the same weights for this to work properly, so some people will even fill up an identical tube with water or something just to add weight where itâs due.
In this pic I made, the red represents a filled tube. You see how if you add it in one hole, you need to add another evenly filled one in the hole across from it. To add a counterbalance. If the centrifuge is unbalanced, itâll start shaking, making weird noises, and can ultimately damage the device.
Your centrifuge will have its own timer, so itâll shut itself off whenever you want. It also has a speed adjustment knob which will control the RPM. The max RPM is 4000, so each knob (beginning at zero), is probably ~666 RPM each.
There are a few different vocab words that you should know about the centrifuge.
RPM: rotations/revolutions per minute.
G-force/RCF: the term g force means gravity force, how much times stronger is the force than normal gravity? A higher g force would mean itâs spinning faster. This is expressed in g (ex: 100g would mean 100 times earths gravity).
Rotor: the rotor is the part of your centrifuge that spins.
Rotor radius: distance from the center. The greater distance from the center, the more increased your RCF will be.
Your collection tubes can range anywhere up until 20ml since thatâs the max amount the zenstyle 800-1 can carry. PRP collection tubes are meant to have anticoagulants present inside them, which means anti clogging basically. Sodium citrate 3.2% and ACD-A are the most popular anticoagulants used for PRP, although ACD-A is superior. However, anticoagulation tubes are not used for PRF, since it defies PRFs purpose.
Kinds of blood fillers:
PRP
This stands for platelet rich plasma. Now what the hell does that mean? Plasma and platelets are both found in your blood. Plasma meaning the yellow liquid base that makes up ~55% of your blood, and platelets meaning blood cells that repair and rejuvenate. Now that thatâs explained, you can guess what this treatment is about.
This treatment is used in orthopedic contexts that help to heal muscles, tendons, and more, but it has also grown popular in the cosmetic industry. PRP injections have been used for collagen stimulation, scar covering, skin rejuvenation, AND hair rejuvenation. It is injected and can also be microneedled into the face, which is called the âvampire facialâ treatment. Using your own blood to regenerate your youthfulness.
- A sample of your blood is drawn (depending on desired treatment area).
- It is spun in a centrifuge to separate its layers.
- The doctor extracts the layer with the most platelets. They might even extract a layer and spin it again to be extra precise.
- They inject it/apply it!
A larger quantity of blood drawn automatically means more platelets you begin with. Lots of people who get this treatment are unsatisfied because their doctors donât even draw enough blood in the first place for it to be classified as âplatelet richâ. The more blood drawn=the more platelets you have.
Watch this whole video.
How is Proper Platelet-Rich Plasma (PRP) Prepared? -- ZengoMD
This guy
speaks about gel plug systems in his video which are âprp kitsâ that contain tubes that have gel in them. This special gel separates each layer after one spin, so it is usually a single spin system. The issue with that (as spoken about in the vid) is when you only do one spin, you only get moderate platelet concentrations. As seen in the kakudo study, double spin systems can produce stronger and actual significant effects on fibroblasts and stem cells. MANY DOCTORS USE THESE GEL KITS UNAWARE OF HOW USELESS THEY ARE BTW. Props to this doctor for exposing their asses. As he said, even if the platelet count in the plasma portion ends up containing only ONE more platelet than the total amount in the baseline, itâs still considered âplatelet richâ 
âThe amount of blood drawn and the way it is handled are the two most important variablesâ
Bottom line is single spin prp wonât give you the results you are hoping for.
Now, the average range of platelets per liter in a human are 150 billion-450 billion. Anything under 150,000 per microliter is not normal, thereâs a condition for it called thrombocytopenia. Iâm gonna be rightfully assuming none of you have it since 2.5% of the population has it.
We will assume you have the bare minimum amount of platelets in your blood, which is 150 billion/liter. That means you have 150 million platelets per ml. This is just an example.
A single spin typically increases platelet concentration to 1.5x baseline concentration, (and in rare cases up to 3x). So after the single spin, youâd have 225 million platelets per ml in the buffy coat layer.
A double spin can further concentrate the platelets, reaching around 5-8x compared to the whole blood. Assuming the lowest again, if it increases the concentration by 5x, you will have 750 million platelets per ml.
To fill in the missing piece, a 30-60ml blood draw is crucial for obtaining optimal results.
You want to end up with a MINIMUM of 5.5 billion platelets PER injection.
Letâs say you fill a 60ml syringe with blood, (assuming you have 150mil platelets per ml of blood, youâd have 9 billion platelets in your syringe). Dividing 9 billion by 5.5billion per injection, youâd have only 1 injection in total (since recovery rates are not perfect, 50-80% is most common). Realistically, youâd have way more than 9 billion platelets in your 60ml sample (since 150mil platelets per ml is the bare minimum), meaning youâd be able to split it up into more injections than just 1. Higher platelet counts will call for a better prp yield, but injection count is constrained by recovery efficiency.
When words like 1.5x are used, itâs talking about 1.5x the baseline concentration, since obviously the platelet count doesnât increase during centrifugation. What centrifugation will do is change the distribution of your platelets and how densely packed they are (water)
Tldr:
-Double spin protocol mogs single spin
-The more blood you draw, the more platelets youâll get
-Anything below 5.5 billion platelets per injection wonât produce optimal results
-Unfortunately, you will have to draw 40-60ccs of blood minimum. Which will be costly.
Watch this whole video.
How is Proper Platelet-Rich Plasma (PRP) Prepared? -- ZengoMD
This guy
speaks about gel plug systems in his video which are âprp kitsâ that contain tubes that have gel in them. This special gel separates each layer after one spin, so it is usually a single spin system. The issue with that (as spoken about in the vid) is when you only do one spin, you only get moderate platelet concentrations. As seen in the kakudo study, double spin systems can produce stronger and actual significant effects on fibroblasts and stem cells. MANY DOCTORS USE THESE GEL KITS UNAWARE OF HOW USELESS THEY ARE BTW. Props to this doctor for exposing their asses. As he said, even if the platelet count in the plasma portion ends up containing only ONE more platelet than the total amount in the baseline, itâs still considered âplatelet richâ âThe amount of blood drawn and the way it is handled are the two most important variablesâ
Bottom line is single spin prp wonât give you the results you are hoping for.
Now, the average range of platelets per liter in a human are 150 billion-450 billion. Anything under 150,000 per microliter is not normal, thereâs a condition for it called thrombocytopenia. Iâm gonna be rightfully assuming none of you have it since 2.5% of the population has it.
We will assume you have the bare minimum amount of platelets in your blood, which is 150 billion/liter. That means you have 150 million platelets per ml. This is just an example.
A single spin typically increases platelet concentration to 1.5x baseline concentration, (and in rare cases up to 3x). So after the single spin, youâd have 225 million platelets per ml in the buffy coat layer.
A double spin can further concentrate the platelets, reaching around 5-8x compared to the whole blood. Assuming the lowest again, if it increases the concentration by 5x, you will have 750 million platelets per ml.
To fill in the missing piece, a 30-60ml blood draw is crucial for obtaining optimal results.
You want to end up with a MINIMUM of 5.5 billion platelets PER injection.
Letâs say you fill a 60ml syringe with blood, (assuming you have 150mil platelets per ml of blood, youâd have 9 billion platelets in your syringe). Dividing 9 billion by 5.5billion per injection, youâd have only 1 injection in total (since recovery rates are not perfect, 50-80% is most common). Realistically, youâd have way more than 9 billion platelets in your 60ml sample (since 150mil platelets per ml is the bare minimum), meaning youâd be able to split it up into more injections than just 1. Higher platelet counts will call for a better prp yield, but injection count is constrained by recovery efficiency.
When words like 1.5x are used, itâs talking about 1.5x the baseline concentration, since obviously the platelet count doesnât increase during centrifugation. What centrifugation will do is change the distribution of your platelets and how densely packed they are (water)
Tldr:
-Double spin protocol mogs single spin
-The more blood you draw, the more platelets youâll get
-Anything below 5.5 billion platelets per injection wonât produce optimal results
-Unfortunately, you will have to draw 40-60ccs of blood minimum. Which will be costly.
When it comes to phlebotomy tubes for this kind of thing, finding a cheap source is practically impossible. But I found this one. It doesnât state anything about containing gel, so fortunately I donât think these use gel. This is the only source I could find for a cheap ADC-A tube/s, but if I ever find more sources I will put them here.
https://ksscientific.com/products/6...variant=44620214730943&utm_source=chatgpt.com
(THIS IS A SIX PACK SO HELL YEAH, THISLL BE ENOUGH TO DRAW 40+ CCs OF BLOOD IF U COULDNâT TELL
âŚbut youâll have to buy this six pack twice to have an extra tube for your second spin. Sorry. Not cheap, I know.)
1) Phlebotomy
Put on gloves. First we need to start off with drawing your blood. Which in my opinion is the most stomach turning part (I literally have to lay down at the doctors when I get mine drawn), but I donât speak for everyone.
You need to assemble part of your materials first. Begin by connecting your butterfly needle to the holder if they arenât already connected.
They should look like this.
The most popular place to draw blood from is inside the elbow, the median cubital vein. A tourniquet is applied to slow down circulation and make the vein more visible.
Wrap a tourniquet about 3 inches above the inside of your elbow, make sure itâs not too tight. It should be easily removable and still allow for blood flow. It shouldnât be on ANY longer than 1 minute and should be taken off when blood starts flowing. After about 30 seconds you may start to see a vein grow enlarged. Once you find a good vein, wipe the area down with an alcohol wipe. Then pinch the butterfly wings and proceed to enter the vein with your needle at an angle of 30° or less. A 21 gauge needle is most commonly used during blood drawings.
While the needle is inserted, you can connect your collection tube inside the holder. The blood starts flowing now.
One by one, you can fill up your tubes. When finished, remove the needle gently and slowly. Apply pressure on the vein with a gauze pad, and secure it with some medical tape.
2) Centrifugation
After your tubes are full, gently invert the tube a couple times. This is done to mix the blood with the anticoagulant.
Now it is time to load them into your centrifuge. Your first centrifugal spin, called the âsoft spinâ, is just supposed to safely separate the layers in your blood sample.
The soft spin is supposed to be 200-400g for 10-15 minutes. Iâve seen SO many different protocols used online since ig thereâs no fixed double spin settings. But Iâm just gonna say 1500 rpm for 10-15 minutes.
After the times up, youâll get your tubes and then extract the upper layer/buffy coat of your prp out with a 3mL syringe and 18g needle. Itâs okay if you get just a tad bit of red blood in your syringe. With that, you will reinject what you have into a different fresh anticoagulant tube.
Now for the second spin/âhard spinâ, youâll use 3500rpm for 5 minutes. Now, youâll have an even more concentrated solution.
Donât forget to add a counterbalance so the centrifuge works properly.
Now you have your platelet rich plasma, and you should extract the ppp layer. Isolating it from your liquid gold. Sometimes people keep a little ppp behind though, which is fine.
People will inject prp as is, and as said before, some do a âvampire facialâ.
That is when you microneedle all over your skin, and spread your prp all over the microneedled areas. Using blood to rejuvenate your own youth, vampire type shi
If you do choose to do this, make sure to apply the prp with sterile gloves on. This is a pdf of aftercare
https://www.cupertinofacialesthetic...s/2019/04/Micro-Needling-Prp-Post-Op-Care.pdf
Injecting/applying your prp immediately and in a 30 minute time window is ideal.
Total equipment:
-prp tubes (buy the same pack 2x for double spin)
-butterfly needle w/ holder
-tourniquet
-pack of 3ml luer lock syringes
-pack of 18g needles
-tube holder
-gauze pads/medical tape for after blood draw
-centrifuge
-sterile gloves
https://ksscientific.com/products/6...variant=44620214730943&utm_source=chatgpt.com
(THIS IS A SIX PACK SO HELL YEAH, THISLL BE ENOUGH TO DRAW 40+ CCs OF BLOOD IF U COULDNâT TELL
âŚbut youâll have to buy this six pack twice to have an extra tube for your second spin. Sorry. Not cheap, I know.)1) Phlebotomy
Put on gloves. First we need to start off with drawing your blood. Which in my opinion is the most stomach turning part (I literally have to lay down at the doctors when I get mine drawn), but I donât speak for everyone.
You need to assemble part of your materials first. Begin by connecting your butterfly needle to the holder if they arenât already connected.
They should look like this.
The most popular place to draw blood from is inside the elbow, the median cubital vein. A tourniquet is applied to slow down circulation and make the vein more visible.
Wrap a tourniquet about 3 inches above the inside of your elbow, make sure itâs not too tight. It should be easily removable and still allow for blood flow. It shouldnât be on ANY longer than 1 minute and should be taken off when blood starts flowing. After about 30 seconds you may start to see a vein grow enlarged. Once you find a good vein, wipe the area down with an alcohol wipe. Then pinch the butterfly wings and proceed to enter the vein with your needle at an angle of 30° or less. A 21 gauge needle is most commonly used during blood drawings.
While the needle is inserted, you can connect your collection tube inside the holder. The blood starts flowing now.
One by one, you can fill up your tubes. When finished, remove the needle gently and slowly. Apply pressure on the vein with a gauze pad, and secure it with some medical tape.
2) Centrifugation
After your tubes are full, gently invert the tube a couple times. This is done to mix the blood with the anticoagulant.
Now it is time to load them into your centrifuge. Your first centrifugal spin, called the âsoft spinâ, is just supposed to safely separate the layers in your blood sample.
The soft spin is supposed to be 200-400g for 10-15 minutes. Iâve seen SO many different protocols used online since ig thereâs no fixed double spin settings. But Iâm just gonna say 1500 rpm for 10-15 minutes.
After the times up, youâll get your tubes and then extract the upper layer/buffy coat of your prp out with a 3mL syringe and 18g needle. Itâs okay if you get just a tad bit of red blood in your syringe. With that, you will reinject what you have into a different fresh anticoagulant tube.
Now for the second spin/âhard spinâ, youâll use 3500rpm for 5 minutes. Now, youâll have an even more concentrated solution.
Donât forget to add a counterbalance so the centrifuge works properly.
Now you have your platelet rich plasma, and you should extract the ppp layer. Isolating it from your liquid gold. Sometimes people keep a little ppp behind though, which is fine.
People will inject prp as is, and as said before, some do a âvampire facialâ.
That is when you microneedle all over your skin, and spread your prp all over the microneedled areas. Using blood to rejuvenate your own youth, vampire type shi
If you do choose to do this, make sure to apply the prp with sterile gloves on. This is a pdf of aftercare
https://www.cupertinofacialesthetic...s/2019/04/Micro-Needling-Prp-Post-Op-Care.pdf
Injecting/applying your prp immediately and in a 30 minute time window is ideal.
Total equipment:
-prp tubes (buy the same pack 2x for double spin)
-butterfly needle w/ holder
-tourniquet
-pack of 3ml luer lock syringes
-pack of 18g needles
-tube holder
-gauze pads/medical tape for after blood draw
-centrifuge
-sterile gloves
PRF
This on the other hand stands for platelet rich fibrin. This isnât like PRP where itâs liquidy, this one is stickier and is used as a filler. Fibrin is a sticky protein found in your blood that almost acts as a net and traps platelets and other blood cells. Itâs used for basically the same things as PRP, but the unique part about it is its filler type consistency. If youâve heard of EZ gel, this is what is used to make it. PRF can also be used for a vampire facial, or just injected by itself.
- A sample of your blood is drawn
- Itâs slowly spun in a centrifuge so the layers separate and a fibrin clot is caused in the middle
- The jelly like clot is picked out from the tube and prepared for use. It can be used as is for an implant type filling, broken down into a gel for filler, or broken down into a liquid for skin.
Something that is essential to creating prf are using completely ADDITIVE FREE glass blood collection tubes. I found these online and they look perfect. This company sells tubes in both plastic and glass, and this listing is luckily glass.
https://wetexmp.com/products/prf-platelet-rich-fibrin?utm_
(I donât know for sure if theyâll let you purchase from them without a medical license tho..)
Itâs a frequent paradox people face where they are convinced the tubes they have are âadditive freeâ when the tops are fucking silicone coated. Plastic tubes generally use silicone coatings which will literally contaminate the blood itself, you do not want silica inside of you.
These are a possibility, although it doesnât say it has no silicone. But at the same time, it doesnât say it has silicone in it.
https://www.ebay.com/itm/1577650545...754&gbraid=0AAAAAD_QDh-_tFj__vBTWqJh2QvI9O1kg
Itâs the cheapest option. If you ever wanted to go the extremely safe route you can go throw hundreds of dollars at the Dr. Choukron glass prf tubes. We know for a fact that these are completely additive free.
But are u gonna buy these? No, cause ur broke.
This looks good too, but remember youâd have to buy multiple (like 3 minimum), so youâd be able to draw atleast 40ccs of blood.
https://dermafusionaesthetics.com/p...523&gbraid=0AAAAA-xyZFTPThGIpSa9IbS4MMNd3-TW4
Overall, if u are looking for prf tubes make sure they are
-glass
-additive free
-silicone free.
-NO ANTICOAGULANT OR GEL OR ANY OF THAT
Anyways, moving on to the first step
1) Phlebotomy
Put on gloves. First we need to start off with drawing your blood. Which in my opinion is the most stomach turning part (I literally have to lay down at the doctors when I get mine drawn), but I donât speak for everyone.
You need to assemble part of your materials first. Begin by connecting your butterfly needle to the holder if they arenât already connected.
They should look like this.
The most popular place to draw blood from is inside the elbow, the median cubital vein. A tourniquet is applied to slow down circulation and make the vein more visible.
Wrap a tourniquet about 3 inches above the inside of your elbow, make sure itâs not too tight. It should be easily removable and still allow for blood flow. It shouldnât be on ANY longer than 1 minute and should be taken off when blood starts flowing. After about 30 seconds you may start to see a vein grow enlarged. Once you find a good vein, wipe the area down with an alcohol wipe. Then pinch the butterfly wings and proceed to enter the vein with your needle at an angle of 30° or less. A 21 gauge needle is most commonly used during blood drawings.
While the needle is inserted, you can connect your collection tube inside the holder. The blood starts flowing now.
One by one, you can fill up your tubes. When finished, remove the needle gently and slowly. Apply pressure on the vein with a gauze pad, and secure it with some medical tape.
2) Centrifugation
Once your tubes are full, load them into the centrifuge properly. As said earlier, this is gonna be a single spin treatment.
Iâve found studies that favor 700g for 8 minutes is recommended for producing solid PRF.
400g for 12 minutes is also recommended. So somewhere along that ratio would be good.
After it is done centrifuging, you should end up with something like this.
Now this is where things branch off. You can either leave it as is, or go even further and make it into EZ gel. A more filler like substance that adds more stability.
Total equipment:
-prf tubes
-pack of 3ml luer lock syringes
-18g needles pack
-butterfly needle w/ holder
-tourniquet
-gauze pads/medical tape for blood drawing
-sterile gloves
-female to female luer lock connector
-dry bath incubator (optional: for ez gel)
-centrifuge
-centrifuge tubes
https://wetexmp.com/products/prf-platelet-rich-fibrin?utm_
(I donât know for sure if theyâll let you purchase from them without a medical license tho..)
Itâs a frequent paradox people face where they are convinced the tubes they have are âadditive freeâ when the tops are fucking silicone coated. Plastic tubes generally use silicone coatings which will literally contaminate the blood itself, you do not want silica inside of you.
These are a possibility, although it doesnât say it has no silicone. But at the same time, it doesnât say it has silicone in it.
https://www.ebay.com/itm/1577650545...754&gbraid=0AAAAAD_QDh-_tFj__vBTWqJh2QvI9O1kg
Itâs the cheapest option. If you ever wanted to go the extremely safe route you can go throw hundreds of dollars at the Dr. Choukron glass prf tubes. We know for a fact that these are completely additive free.
But are u gonna buy these? No, cause ur broke.
This looks good too, but remember youâd have to buy multiple (like 3 minimum), so youâd be able to draw atleast 40ccs of blood.
https://dermafusionaesthetics.com/p...523&gbraid=0AAAAA-xyZFTPThGIpSa9IbS4MMNd3-TW4
Overall, if u are looking for prf tubes make sure they are
-glass
-additive free
-silicone free.
-NO ANTICOAGULANT OR GEL OR ANY OF THAT
Anyways, moving on to the first step
1) Phlebotomy
Put on gloves. First we need to start off with drawing your blood. Which in my opinion is the most stomach turning part (I literally have to lay down at the doctors when I get mine drawn), but I donât speak for everyone.
You need to assemble part of your materials first. Begin by connecting your butterfly needle to the holder if they arenât already connected.
They should look like this.
The most popular place to draw blood from is inside the elbow, the median cubital vein. A tourniquet is applied to slow down circulation and make the vein more visible.
Wrap a tourniquet about 3 inches above the inside of your elbow, make sure itâs not too tight. It should be easily removable and still allow for blood flow. It shouldnât be on ANY longer than 1 minute and should be taken off when blood starts flowing. After about 30 seconds you may start to see a vein grow enlarged. Once you find a good vein, wipe the area down with an alcohol wipe. Then pinch the butterfly wings and proceed to enter the vein with your needle at an angle of 30° or less. A 21 gauge needle is most commonly used during blood drawings.
While the needle is inserted, you can connect your collection tube inside the holder. The blood starts flowing now.
One by one, you can fill up your tubes. When finished, remove the needle gently and slowly. Apply pressure on the vein with a gauze pad, and secure it with some medical tape.
2) Centrifugation
Once your tubes are full, load them into the centrifuge properly. As said earlier, this is gonna be a single spin treatment.
Iâve found studies that favor 700g for 8 minutes is recommended for producing solid PRF.
400g for 12 minutes is also recommended. So somewhere along that ratio would be good.
After it is done centrifuging, you should end up with something like this.
Now this is where things branch off. You can either leave it as is, or go even further and make it into EZ gel. A more filler like substance that adds more stability.
Take your tube of PRF, and you will use a syringe to suck up the PPP layer/albumin rich layer (which is like 2/3 of the top layer). Whilst leaving the buffy coat layer (PRF) with the growth factors and platelets behind.
You will then take the PPP and heat it up. By doing this, you will be reshaping its protein structure and making it into a thicker gel. Depending on how firm you want this filler to be, it is heated from 70-90°C for 10-15 minutes. The standard is usually 70° for 15 minutes. Now how are we supposed to heat this up? Well, clinicians use different devices like bath incubators and heat blocks. Youâre probably thinking this is yet ANOTHER piece of expensive medical grade equipment, how the hell can someone afford this?!
Youâre in luck because I found one here by onilab.
(Order the 5mL block one)
https://onilabus.com/products/onila...variant=45092780015912&utm_source=chatgpt.com
This is also another place
https://www.4es-usa.com/products/bl...variant=43284446249053&utm_source=chatgpt.com
To create the perfect fit and ensure for maximized thermal contact, squirt your albumin rich layer from your syringe into a centrifuge tube. Now itâs ready to be loaded into the heating block
https://www.american-hospitalsupply...621&gbraid=0AAAAABTKktTS0J9igtEe9UHLSX9Jcpahq
With the device, you can set your temperature and timing. Put your centrifuge tubes in. (Ignore that the photo below is using syringes)
Once the times up, the liquid in the tube will have a lighter/cloudier type look to it. This is due to all the proteins denaturing from the heat. Youâve got to take it out of the heating block and let it sit and cool down for 5-10 mins. Then after, draw it back up from the tube into your syringe.
Now youâve got your filler like substance, and the next step is to combine it with your liquid PRF.
You will do this by using a standard female to female luer lock connector, and connecting your albumin rich gel with your liquid PRF. You will then pass the fluids between syringes, combining them both to create the final product. Smoothening out clumps, and creating an even consistency. 10-15 transfers.
After that, you are done, and have finished your EZ gel. It should be injected in the next 20 minutes since it will harden overtime.
You will then take the PPP and heat it up. By doing this, you will be reshaping its protein structure and making it into a thicker gel. Depending on how firm you want this filler to be, it is heated from 70-90°C for 10-15 minutes. The standard is usually 70° for 15 minutes. Now how are we supposed to heat this up? Well, clinicians use different devices like bath incubators and heat blocks. Youâre probably thinking this is yet ANOTHER piece of expensive medical grade equipment, how the hell can someone afford this?!
Youâre in luck because I found one here by onilab.
(Order the 5mL block one)
https://onilabus.com/products/onila...variant=45092780015912&utm_source=chatgpt.com
This is also another place
https://www.4es-usa.com/products/bl...variant=43284446249053&utm_source=chatgpt.com
To create the perfect fit and ensure for maximized thermal contact, squirt your albumin rich layer from your syringe into a centrifuge tube. Now itâs ready to be loaded into the heating block
https://www.american-hospitalsupply...621&gbraid=0AAAAABTKktTS0J9igtEe9UHLSX9Jcpahq
With the device, you can set your temperature and timing. Put your centrifuge tubes in. (Ignore that the photo below is using syringes)
Once the times up, the liquid in the tube will have a lighter/cloudier type look to it. This is due to all the proteins denaturing from the heat. Youâve got to take it out of the heating block and let it sit and cool down for 5-10 mins. Then after, draw it back up from the tube into your syringe.
Now youâve got your filler like substance, and the next step is to combine it with your liquid PRF.
You will do this by using a standard female to female luer lock connector, and connecting your albumin rich gel with your liquid PRF. You will then pass the fluids between syringes, combining them both to create the final product. Smoothening out clumps, and creating an even consistency. 10-15 transfers.
After that, you are done, and have finished your EZ gel. It should be injected in the next 20 minutes since it will harden overtime.
Total equipment:
-prf tubes
-pack of 3ml luer lock syringes
-18g needles pack
-butterfly needle w/ holder
-tourniquet
-gauze pads/medical tape for blood drawing
-sterile gloves
-female to female luer lock connector
-dry bath incubator (optional: for ez gel)
-centrifuge
-centrifuge tubes
I was thinking about adding a PRFM one, but calcium chloride 10% is unfortunately not available to buy anywhere
Iâm not a medical professional, this post is not encouraging anybody to replicate what Iâve said. The information here is all purely based off research,
@Chainsandwhips2 @arya @TonyDr @genio @foidslayer @Histy @LaWi @ecoli
